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KMID : 0903620040450060287
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Journal of the Korean Society for Horticultural Science
2004 Volume.45 No. 6 p.287 ~ p.292
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Phenotypic and Molecular Analysis of Mutants Generated with Ion Beam in Tobacco (Nicotiana tabacum)
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Kim Young-Hee
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Abstract
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The anthers of tobacco (Nicotiana tabacum cv. Bright Yellow-4) were irradiated with ¹⁴N-heavy ion beam at 5 Gy, and then haploids were selected in M₁ generation (pale-pink flower color). The haploids of M₁ generation were self-pollinated and flower color mutants were selected from M₂ generation. The phenotypic and molecular differences were observed between the mutants and the non-mutants. The petals of the mutants were rounder in shape and had much lighter color compared to those of non-mutated ones. The dot blotting and Northern hybridization analysis revealed that the chalcone synthase (CHS) gene in flower tissues was less expressed in the mutants than in the non-mutants. This implies that the expression of CHS gene in the anthocyanin biosynthesis pathway could be suppressed in mutant plants.
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KEYWORD
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anthocyanins, chalcone synthase, ¹⁴N-heavy ion beam
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